Ethno-medicinal profile of Different Plant Parts of Calotropis procera (Ait

Ethnobotanical Leaflets 14: 721-42, 2010.

Ethno-Medicinal Profile of Different Plant Parts of Calotropis procera (Ait.) R. Br.

Raginee Verma, G.P. Satsangi and J.N. Shrivastava

Microbiology Lab., Department of Botany, DEI, Dayalbagh, Agra, India

e.mail: janendra.srivastava@gmail.com

ragini26verma@gmail.com

gurupsatsangi@gmail.com

Issued: July 01, 2010

Abstract

The present paper reviews the literature on recent ethno medicinal uses of every plant part of Calotropis procera (1968-2009) and its medicinal properties used for the treatment of various ailments as in the case of many types of fevers, rheumatism, indigestion, cough, cold, eczema, asthma, elephantiasis, nausea, vomiting and diarrhea, etc. The review includes accounts of medicinal values of all parts of the plant that have been used in folk medicine as a remedy. The name and parts of the plant studied, the spectrum of activity, and methods used are discussed in this review paper.

Key words: Calotropis procera; traditional medicines; ethno-medicinal use.

Introduction

The herbal medicines occupy distinct position right from the primitive period to present day. The ethnobotanical pharmacology is as old as man himself. These medicines have less side effects and man can get the herbs easily from nature. India being a tropical country is blessed with vast natural resources and ancient knowledge for its judicious utilization. However, in order to make these remedies acceptable to modern medicine, there is a need to scientifically evaluate them, to identify the active principles and to understand the mechanism of action (Ashok Vaidya, 1998)^.Calotorpis procera in India holds a pride of place largely because of its other uses and economic values. The genus Calotropis R.Br. (Asclepiadaceous) is distributed in tropical and subtropical regions of Asia and Africa (The wealth of India, 1959). It is represented in India by two species viz. C. procera and C. gigantean.

Calotropis procera (Ait.) R. Br., a wild growing plant of family Asclepiadaceae, is well known for its medicinal properties. Different parts of this plant have been reported to exhibit anti-inflammatory, analgesic, and antioxidant properties. It is found in most parts of the world in dry, sandy and alkaline soils and warm climate and is more common in south western and central India and western Himalayas. It is found in waste lands and grows as a weed in agricultural lands. In ancient Ayurvedic medicines the plant Calotropis procera was known as “Rakta arka”.

Morphologically the plant is erect, tall, large, much branched and perennial shrub or small tree that grows on a height of 5.4m, with milky latex throughout. Bark is soft and corky, branches stout, leaves sub sessile, opposite, decussate, broadly ovate, oblong, elliptic or obovate, acute, thick, glaceous, green coloured with fine cottony pubescent hair on young. Flowers in umbellate cymes and tomentose on young. Seeds broadly ovate, acute, flattened, minutely tomentose, brown coloured and silky.

Methodology

Most of the research papers, research articles and review papers were consulted and compiled. The useful material regarding the information of ethno medicinal aspects of C. procera were collected from time to time and summarized in present paper. This paper recovers the traditional medicinal values of each and every part of the selected the plant (C. procera).

Result

A number of research papers, articles and review papers treat the ethno medicinal aspects of this plant. Table 1, 2 and Fig. 1 showing the percentage of ethnomedicinal uses of different plant parts of C. procera.

Table 1. Ethno medicinal uses of different plant parts of C. procera.

S.No.

Part used

Preparation

Use

Refrences

10.

11.

12.

13.

14.

15.

16.

17.

18.

19.

20.

21.

22.

23.

24.

25.

26.

27.

28.

29.

30.

31.

32.

33.

34.

35.

36.

37.

38.

39.

40.

41.

42.

43.

44.

45.

46.

47.

48.

49.

50.

51.

52.

53.

54.

55.

56.

57.

58.

59.

60.

61.

62.

63.

64.

65.

66.

67.

68.

69.

70.

71.

72.

73.

74.

75.

76.

77.

Whole Plant

Whole plant

Whole Plant

Root

Roots

Root

Root bark

Root Bark

Stem

Stem latex

Stem bark

Leaves

Leaf latex

Latex

latex

Latex

Flower

Stigma

Seed

In Nigeria traditional medicine, C. procera is either used alone or with other herbs

Whole plant is used

The whole plant is used

Ash of whole plant

The alcoholic extracts of the root and leaves of C. procera

Root used as

Chloroform root extract of Calotropis procera

Root is used as

Root are used to treat

Roots are tied with the help of a red thread on the affected part

Extract of root is taken orally by the tribal ladies.

Used as purgative and taken in

The root powder is mixed with butter and this ointment is applied to.

given with black pepper

The paste of root bark

The root bark powder is used.

Root bark powder

The secretions from the root bark are traditionally used

The bark of root is taken out and mixed with a minute dose of arsenic and given in the form of a pill

The bark of root is powdered

Stem used as tooth brush

Stem is used as Maswak

Latex is mixed with Sodium Chloride (Nacl) and warmed on hot slow heating. During heating continuous stirring should be done. One microgram given orally thrice a day.

The latex applied locally During the course of this treatment, prepared from the whole plant Swertia Chirayita is also taken.

4 gm of latex is taken orally by the tribal’s

Small pieces (stem bark) in maceration in a liter of water,

Dried powered leaves can be dusted over wounds, ulcers and old sores

In morning, before sunrise, tender leaves were given in a capsule with water on an empty stomach. After treatment for three days

Leaf extract, chopped leaves and latex of C.procera have also shown great promise

Mature leaves of C. procera along with urine of the patient concerned are filled in an air tight earthen pot. After ten days, a cloth wet in this urine is applied on

Leaves of Calotropis procera are used

Leaves used as

Leaf extracts mixed with oil on heat.

Leaves are smoked.

Leaf and black pepper used

leaves are used to treat

Young leaves are crushed and the juice is expressed on the palms of the person venomated. It is allowed to be there for five minutes and is sniffed. If immediately sneezing starts the snake is declared to be poisnous.

Milk of goat feeding on leaves of C. procera is given to infants

The fresh leaves of the plant are warmed and are applied as poultice

The decoction (leaves)

Leaves are pounded with castor oil and banded over knee joints

The leaves are heated and bandage is made.

The leaves are used

Fresh leaves are roasted in the ghee or oil and applied on the

The leaves and flowers are crushed and the paste is mixed with honey

leaves mixed with turmeric, honey and karanji was applied as a paste

Powder of 5g dried leaves mixed with gur given orally before sunrise for 5 days.

Leaves are used on

Leaf latex is externally applied twice a day for 2-3 days

Leaf latex is used to

Small quantity of fresh latex is applied over

Leaf latex is used as antidote

Calotropin isolated from latex is used as a remedy for

Latex is antisyphilitic and is also

Milky latex is locally applied in the treatment of

Preparations from latex with honey are used as

Latex is applied

Latex is, either taken internally or locally to the mouth of uterus with the aid of a stick well coated with juice.

A single dose of the aqueous suspension of the dried latex was effective to a significant level against the

Topical application of 20 micro liters of 1% sterile solution of the latex of Calotropis procera twice daily for 7 days

Pills of a black gram size are made from one tablespoonful of latex mixed with 20g sugar and 1g calcium carbonate. One pill twice a day given for three days is said

Latex of the plant is filled in spaces between nails and finger tips of patient twice daily for a few days to cure conjunctivitis.

Latex is used in the treatment of.

Latex is applied

Latex is used

Take 26 gm of lahori salt, put it in an earthen pot, add Calotropis milk so that the salt dipped in to it, and cover earthen pot mouth and heat up. Grind it and use the powder externally for gum bleeding with small amount of HCL. It is effective for

4 to 5 drops of fresh latex of the plant is dropped over the injured portion as an antiseptic in the

Milky latex is used to treat

Used as

Latex is topically used as

The milky juice is poisonous and is used in

Latex of Calotropis procera and Mangifera indica mixed with one drop of conc. HCL is applied locally as an

Milky latex of plant is applied on inflamed areas to

Latex and leaves mixed with turmeric, honey and karanji was applied as a paste.

Latex is used in

Extracts of Calotorpis procera flower was investigated for

Pills of the size of a black gram are made from the paste obtained by mixing 6gm flower buds with 7-8 black pepper seeds and 3gm salt. Two pills are given twice daily for three days.

Flowers of C. procera and leaves of Nicotiana tobacum L. (Solanaceae) taken in equal quantity, are burnt to ash, which is inhaled

Internal part of flower and sugar used for

The hair is washed with flowers

Powder of dried flowers of Calotropis procera grind along with Papaver somniferum and Eletteria cardomomum (20gm each), called Tally, used three times daily (about one teaspoonful)

The leaves and flowers are crushed and the paste is mixed with honey

Flowers of this plant are used

About 12gm stigmas are added to 5-8 black pepper, 3gm black salt and opium (equal to one seed of mustard) and make into pills of the size of black pepper. One pill three times daily to given with hot water for three times a days

Take 3 gm of Calotropis seeds, 18 gm of red chili seeds and 6 gm opium. Grind all of these and mix it in one teaspoon of ginger and half cup of onion water, if unavailable then use syrup of sugar and citrus medica water..

To treat common diseases such as fever, rheumatism, indigestion, cold, eczema and diarrohea.

In boils and also to remove thorn from body.

for the treatment of jaundice

Is used as coloring material.

Were found to have anticanceractivity against human epidermal carcinoma of the nasophyrnx tissue culture.

digestive agent

Induced acute and chronic liver injury by carbon tetrachloride.

Hydrocede, in headache, severe body pain, malarial fever and convulsion

Eczema, leprosy, elephantiasis, asthma, cough and rheumatism.

To relieve filarial

in dysmenorrhea

Dysentery.

rabid dog bite and on the paralyzed limbs

protracted labour and also used for spleen complaints, elephantiasis, rheumatism,

Is locally applied in elephantiasis.

In the treatment of Diarrhoea and dysentery. In case of diarrhoea it changes the faecal matter into a semisolid mass with in the first day of treatment

Is used to treat diarrhea and dysentery and it is an excellent substitute for ipecac. Traditionally it is used to treat cholera, extracting guinea worms and indigestion.

For the treatment of skin diseases, enlargements of abdominal viscera and intestinal worms.

To people suffering from leprosy.

Used as tonic, antispa modic, expectorant and in large doses emetic.

To enhance amylase activity.

As Tooth brush having the property of curing toothache

To the patient of tuberculosis

To cure Leucoderma.

As an antidote in rabies

Used as tonic and stimulant.

To prompt healing.

All patients suffering from migraine headaches got relief.

As a nematicide in vitro and in vivo.

Bones/ parts affected from Sinus fistula.

To treat fever

Hydrocede, headache, severe body pain, malarial fever and convulsion.

used for joints and waist pain

for asthma

To cure malarial fever.

Eczema, leprosy, elephantiasis, asthma, cough and rheumatism.

To confirm whether the snake that had bitten the person was poisonous or not,

To cure asthma.

In rheumatism, gout and to relieve pains.

Against rheumatism, asthma and also used as sedative.

To get relief from joint pain.

To apply on ulcers

For washing cloths.

Swelling part of body.

To cure flatulence, anorexia, indigestion and intestinal worm infestation.

On the wounds.

To cure migraine

Sores, skin diseases, inflammation and rheumatic joints.

To treat pain in any part of the body.

Treat scorpion and snake bite. Leaf latex is applied on bitten area.

The wart affected area.

For Scorpion bite. Fresh leaves are cut and the excluding latex applied to affected area.

Black scars on face; boils, cold, cough, asthma, ear ache, eczema, skin eruptions, inflammatory lesions, pain of the body, rheumatism, syphilis, leprosy and oedema.

Inserted in to painful tooth cavities and applied to various skin complaints.

Cutaneous diseases such as ringworm, syphilitic sores and leprosy.

Anti rabies and also in the treatment of toothache and cough.

On ringworm and eczema, affected area becomes black after the application due to its burning effect

Used for abortion

Acute inflammatory response.

For wound healing potential.

To cure arthritis.

Blackleg by Zay people

In toothache

As antiseptic

gum bleeding and salt

Skin infection.

Cholera and leprosy.

Purgative and used in dysentery.

Odontalgic.

Various skin diseases also act as purgative.

Antidote in scorpion bite.

Relieve inflammation and snake bite to neutralize poison.

on the wounds

Tooth ache, ringworm and also for removing face darkness.

Cytotoxicity of human colorectal carcinoma cell line and displayed the strong cytotoxic activity

In malaria fever

To get relief from migraine.

Abdominal diseases and asthma.

To remove dandruff from the hair.

To cure cholera and severe dysentery.

To cure flatulence, anorexia, indigestion and intestinal worm infestation.

In piles and asthmatic problems.

To treat cholera.

It is useful in cholera

Kew (1985)

Rai et al (2000)

Jan et al (2009)

Zabihullah et al (2006), Jan et al (2008)

Dhar et al

( 1968)

Mishra and Fridowich (1972)

Basu et al

(1992)

Ajibade et al (2005)

Joshua (2006)

Sen and Behra (2007)

Jain et al (2007)

Showkat (2007)

Khan (2009)

Kumar (2009)

Chopra et al (1983)

Jain et al (1985)

Parrota (2001)

Jasrai et al (2003)

Jan et al (2008)

Mishra and Fridowich (1972)

Zabihullah et al (2006), Jan et al (2008)

Jain et al (2007)

Fatima (2007)

Abdullah(1975),

Awan et al (1986), Said et al (1996)

Prasad (1985)

Khirstova and Tissot (1995)

Anis et al (2000)

Ahmad and Beg (2001)

Ajibade et al (2005)

Shah et al (2006)

Joshua (2006)

Bhogaonkar et al (2007)

Jain et al (2007)

Dhiman (2007)

Fatima (2007)

Reddy (2008)

Jan et al (2008)

Shah et al (2009)

Khan et al (2009)

Patil et al (2009)

Bhatt et al (2009)

Kumar (2009)

Maliya (2007)

Muthuswami and Solomon (2009)

Flatie et al (2009)

Misra and Fridowick (1972)

Mortan (1981)

Kew (1985)

Badruzzamana et al (1989)

Pandey and Anita (1990)

Kumar and Basu (1994)

Rasik et al (1999)

Anis et al (2000)

Giday (2001)

Negi et al (2002)

Ahmad et al (2004)

Khan and Kamran (2006)

Galav et al (2007)

Kumar et al (2007)

Showkat (2007)

Fatima (2007)

Jan et al (2008)

Jain et al (2008)

Khan (2009)

Patil et al (2009)

Kumar (2009)

Smith et al (1995)

Anis et al (2000)

Shah et al (2006)

Jan et al (2008)

Khan (2009)

Kumar (2009)

Anis et al (2000)

Khan and Kamran (2006)

Table 2. Percentage of ethno medicinal uses of different plant parts of C. procera against total number of uses.

PARTS USED

NUMBER OF USES

PERCENTAGE OF USES

WHOLE PLANT
ROOT
ROOT BARK
STEM
STEM LATEX
LEAF
LEAF LATEX
LATEX
FLOWER
STIGMA
SEED

5.19

12.98

7.79

2.59

24.67

3.89

28.57

9.09

1.29

Fig.1. Graphical presentation of ethno medicinal uses of different plant parts of C. procera.

Fig.2. Graphical presentation of ethno medicinal uses of different plant parts of C. procera.

Discussion

This ethno-medico-botanical study on the plant Calotropis procera has revealed the enormous diversity of its medicinal uses and popular use of the plant C. procera for a wide range of common ailments like fevers, rheumatism, indigestion, cough, cold, eczema, asthma, elephantiasis, nausea, vomiting and diarrhea. Either the whole plant or a plant part used singly or mixed with other plant materials to enhance the efficacy.

Plant based drugs have been in use against various diseases since the time immemorial. The primitive man used herbs as therapeutic agents and medicament, which they were able to procure easily. The nature has provided plant wealth for all living creature, which possess medicinal virtues (Bhatti et al, 1998). Medicinal plants are an important source of drugs in traditional system of medicine (Sher and Hussain, 1998a). They are valuable natural resources and regarded as potentially safe drugs. In addition, they are playing an important role in alleviating human suffering by contributing herbal medicines in primary health care system of rural and remote areas where more than 70% of population depends on folklore and traditional system of medicines. The reason for their popularity is due to high cost of allopathic medicines and side effects.

Medicinal plants have been used since prehistoric period for the cure of various diseases. Since these are in common use by the local people and are of great importance that’s why a lot of people are engaged in the trade of important medicinal herbs throughout the world (Elisabetsky, 1990). Especially, people living in villages have been using indigenous plants as medicines since ages because this knowledge transfers from generation to generation and is based on life long experiences. Besides, the villages are far away from cities and mostly lack proper health facilities (Shinwari and khan, 2000).

This field is well established and a lot of work has been done worldwide. Radhakrishman et al (1998) reported ethnobotanical information on Ulteria salicifolia, a monotypic species endemic to south Western Ghats of peninsular India and gave its taxonomic identity, distribution pattern and affinity to an allied genus for the first time. Beyra et al (2004) carried out an ethnobotanical survey from Camaguey, Cuba and reported 111 plant species belonging to 96 genera and 55 families from the study area. These species are used in the treatment of 173 local health problems in the study area. Bondya & Sharma (2004) conducted a survey of medicinal plants used in diabetes in Jharkhand and collected 11 plant species with remarkable uses. Buckingham (1991) reported that there are total of 2,50,000 species of flowering plants in the world, much less than animal species (5-10 million) however, plants contribute to our lives more than animals mainly due to their extra ordinary array of diverse classes of biochemicals with a variety of biological activities. Ji et al (2004) reported the medico-ethnobotany of Nujiang, Northwest Yunnan, and China. They described 52 medicinal plant species belonging to 32 families used for the treatment of various human ailments. Among them, 11 species were reported as rare and 16 were commercially utilized.

The indigenous traditional knowledge of herbal plants of communities where it has been transmitted orally for many years is fast disappearing from the face of world due to transformation of traditional culture. The people, who are native to the area in which the plants occur, use around 90% of the medicinal species (Baquar, 1989). This is indicative of the vast repository of knowledge of plant medicine that is still available for global use, provided of course that it does not get lost before it can be tapped or documented. Traditional and indigenous medical knowledge of plants, both oral and codified, are undoubtedly eroding (Mujtaba and Khan, 2007). In the present scenario, traditional knowledge system in our country is fast eroding and there is an urgent need to inventoried, record all ethno-botanical and cultural information among the diverse ethnic communities before the traditional cultures are completely lost. Therefore, documentation of information on ethno-medicinal uses will help in conserving the knowledge. A comprehensive database of the plants used for various purposes could be saved for the forthcoming generations.

Conclusion

This information about medicinal values of C. procera has paramount importance in life and how these ethno herbal data have key role in life. Moreover, it can be initiative for further phytochemical and pharmacological investigations about the medicinal use of the plant, which may be a step ahead towards the new drug development.

References

1. Abdullah, H.M. 1975. Pakistan Wa Hindustan Ki Jari Bootian, Idar-e-Matbout-C- Sulemani Urdu Bazar Lahore, 29-67.

2. Ahmad, H., Ghulam R.B. and Latif, A. 2004. Medicinal Flora of the Thar Desert: An overview of problem and their feasible solutions. Zonas Aridas N⁰8, Pakistan publishers.

3. Ahmad, I., Beg, Z.A. 2001. Antimicrobial and phytochemical studies on 45 Indian Medicinal Plants against multidrug resistant human pathogens, J. Ethnopharmacology, 74, 13-123.

4. Ajibade, L.T., Fatoba, P.O., Raheem, U.A. and Odunuga, B.A. 2005. Ethno medicine and primary health care in Iiorion, Nigeria, Indian J. traditional knowledge, 4(2): 150-158.

5. Anis, M. Sharma, M.P. and Muhammad, I. 2000. Herbal ethno-medicine of the Gwaliar Forest Division in Madhya Pradesh, India, Pharmaceutical Biology, 38(4): 241-253.

6. Awan, H.M. 1986. Kitab-Mufridat Al Ma'roof Khawasul Adwiyat, Sheikh Ghulam Ali & Sons Ltd., Karachi, pp. 59-60.

7. Badruzzaman, S.M., Alam, M.M. and Wazahat, H. 1989. Traditional treatment of skin diseases in U.P., India, Economic Botany, 43(4): 480-486.

8. Baquar, S.R. 1989. Medicinal and Poisonous Plants of Pakistan. Printas Karachi, Pakistan, pp. 343-344.

9. Basu, A., Sen, T., Ray, R.N. and Nag, A.K. Chaudhuri 1992. Hepatoprotective effects of Calotropis procera root extract on experiment liver damage in animals, Fitoterpia, 63, 507-514.

10. Beyra, A. Del, M., Leon, C., Iglesias, E., Ferrandiz, D., Herrera, R., Volpato, G., Godinez, D., Guimarais, M. and Alvarez, R. 2004. Ethnobotanical studies of medicinal plants in the province of Camaguey (Cuba), Anales Del Jardin Botanico De Madrid, 61(2): 185-203.

11. Bhatt, D., Joshi, G.C. and Tiwari, L.M. 2009. Culture habitat and ethnomedicinal practices by bhotia tribe people of Dharchula region of Pithoragarh District in Kumaun Himalaya, Uttrakhand, Ethnobotanical leaflets, 13, and 975-83.

12. Bhatti, G.R., Qureshi, R. and Shah, M. 1998. Ethnobotany of Calotropis procera with especial reference to the people of Nara Desert, Scientific Sindh, Pub. Salu, Khairpur, 5: 13-22.

13. Bhogaonkar, P.Y. and Kadam, V.N. 2007. Herbal antidotes used for snakebite by banjara people of Umarkhed region in Maharashtra, India, Ethnobotany, 19, 71-78.

14. Bondya, S.L. and Sharma, H.P. 2004. Ethnobotanical studies on plants used in diabetes (Madhumeha) under the Baharagora block of Jharkhand, Ethnobotany, 16(1/2): 139-140.

15. Buckingham, J. 1991. Dictionary of natural compounds, Chapman and Hall U.K., 14-20.

16. Chopra, T., Abrol, B.K. & Handa, K.L. 1983. Medicinal Plants of the arid zone -t Today and tomorrows, printers and publishers, New Delhi, 25.

17. Dhar, M.L., Dhar, M.M., Dhawan, B.N., Mehrotra, B.N. and Ray, C. 1968. Screening of Indian medicinal plants for biological activity, Part I, Ind. J. Exp. Biol. 6, 232-247.

18. Dhiman, A.K. 2007. Conservation of some useful medicinal plants of Haridwar District in Uttaranchal State. Medicinal plants: Conservation, Cultivation and Utilization, 147-166.

19. Elisabetsky, 1990. Plants used as analgesics by amazonian cabocols, Int. J. crude drug research, 28, 309-320.

20. Fatima, M.D. 2007. Patricia Franca De Freitas, Jose Maria Barbosa Filho, Synopsis of the plant known as medicinal and poisnous in northeast of Brazil, Brazilian Journal of Pharmacognosy, 17(1): 114-140.

21. Flatie, T., Gedis, T., Asres, K. and Mariam, T.G. 2009. Ethnomedicinal survey of Berta ethnic group Assosa zone, Benishangul- Gumaz regional state, mid West Ethiopia, J. Ethnobiology and Ethomedicine, 5:14 .

22. Galav, P.K., Nag, A. and Katewa, S.S. 2007. Traditional herbal Veterinary medicines from Mount Abu, Rajsthan, Ethnobotany, 19, 120-123.

23. Giday, M. 2001. An ethnobotanical study of medicinal plants used by the Zay people in Ethiopia, CMB: Skriftserie, Uppasala, 3, 81-99.

24. Jain, Anita. Katewa, S.S., Galav, P.K. and Nag, Ambika 2007. Unrecorded ethnomedicinal uses of biodiversity from Tadgarh Raoli wildlife sanctuary, Rajsthan, India, Acta Botanica Yunnanica, 29 (3):, 337- 344.

25. Jain, Anita, Katewa, S.S., Galav, Praveen and Nag, Ambika, 2008. Some therapeutic uses of biodiversity among the tribals of Rajasthan, India, J. traditional Knowledge, 7 (2): 256-262.

26. Jain, P.K., Verma, R., Kumar, N. & Kumar, A. 1985. Clinical trial of Arka- Mulatwak-Bark of C. procera -a preliminary study, J. Res. Ayur. Sidha 6, 88 -91.

27. Jain, P.K., Kumar, N., Verma, R. 1985. Clinical trials of Arka Mula Tuvaka, bark of Calotropios procera Ait. (R.Br.) on atisar and Pravihika- A preliminary study, J. research in Aurveda and Siddha, 6, 89-91.

28. Jan, G., Khan, M.A. and Gul, F. 2009. Ethnomedicinal plants used against jaundice in Dir Kohistan Valleys (NWFP), Pakistan, Ethnobotanical leaflets, 13, 1029-41.

29. Jan, S., Ajabkhan, M., Uddin, S., Murad, W., Hussain, M. and Ghani, A. 2008. Herbal Remidies used for Gastrointestinal Disorders Kaghan Valley, Nwep, Pakistan, Pak. J. Weed Sci. Res., 14(3-4): 169-200.

30. Jasrai, Yogesh, T., and Chaplot, B.B. 2003. Traditioal Knowledge on plant conservation linked to Beliefs and Religious Rites, In Asian Agri History, Asian Agri History foundation, 319-325.

31. Ji, H., Shengji, P. and Chunlin, L. 2004. An ethnobotanical study of medicinal plants used by the Lisu people in Najiang, Northwest Yunnan, China, Econ. Bot., 58 (Suppl.), 253-264.

32. Joshua, Kayoed. 2006. Conservation of indigenous medicinal botanicals in Ekiti State, Nigeria, J. Zhejiang University Science B, 7(9): 713-718.

33. Kew, F. 1985. The useful plants of west tropical Africa, Vol. (1), Families A-D Edition 2 (Ed Burkill, H.M.) Royal Botanical Gardens, 219-222.

34. Khan, B. and Kamran, S.H. 2006. Community Based Forest conservation in Upper DIR District, NEWP, and Final progress report, Nov. 2005- Oct 2006.

35. Khan, F.M. 2009. Ethnoveterinary medicinal usage of flora of greater Cholistan Desert Pakistan, Pak. Vet. J., 29(2): 75-80.

36. Khirstova, P. and Tissot, M. 1995. Soda Anthroqinone pulping of Hibiscus sabdariffa (Karkadeh) and C.procera from Sudan, Bioresource Technology, 53: 672-677.

37. Kumar, Ashwini 2009. Some important Medicinal plants from different regions of Rajasthan, Scientific Biology, Science.

38. Kumar, V.I., Basu, N. 1994. Anti-inflammatory activity of the latex of Calotropis procera, J. Ethnopharmacology, 44, 123-125.

39. Kumar, V.L., Arya, S. 2007. Medicinal uses and pharmacological properties of Calotropis procera, In: Govil J.N. Editor, Recent Progress in Medicinal Plants, Houston, Tex, USA: Studium Press, 11, 373–388.

40. Maliya, S.D. 2007. Traditional fruit and leaf therapy among Tharus and indigenous people of district Bahraich, India, Ethnobotany, 19, 131-133.

41. Mishra, H.P. and Fridowich, I. 1972. The role of super oxide anion in the autooxidation of epinephrine and a simple assay for superoxide dismutase, J. Biol. Chem., 247, 3170-3185

42. Morton, J.F. 1981. Medicinal plants of Middle America, Springfield, Illinois: Charles, C. Thomas.

43. Mujtaba, G., Khan, M.A. 2007. Check list of medicinal plants of siran valley mansehra-pakistan. Leaflet Quaid-I-Azam University, Islamabad-Pakistan, pp. 15.

44. Muthuswamy, R. and Abay, S.M. 2009. Ethnomedicinal Survey of folk drugs used in Bahirdar Zuria district, Northwestern Ethiopia, Indian J. Traditional Knowledge, 8(2): 281-284.

45. Negi, C.S., Navtiyal, S., Dasila, L., Rao, K.S. and Maikhuri, R.K. 2002. Ethnomedicinal plant uses in a small tribal community in a part of central Himalaya, India, J. Hum. Ecol. 14 (1): 23-31.

46. Bhogaonkar, P.Y. and Kadam, V.N. 2007. Herbal antidotes used for snakebite by Banjara people of Umarkhed region in Maharashtra, India. Ethnobotany 19: 71-78.

47. Pandey, B.P. & Anita 1990. Economic Botany, S. Chand & Company Ltd., Ram Nagar, New Delhi-ll0055, 169-292.

48. Parrota, J.A. 2001. Healing of plants of Penninsular India, AB international Wallingford, U.K., 944.

49. Patil, S.B., Naikwade, N.S., Kondawar, M.S., Magdum, C.S. and Awale, V.B. 2009. Traditional uses of plants for wound healing in the Sangli District, Maharashtra, I. J. Pharm. Tech. Research. 1(3): 876-878.

50. Prasad, G. 1985. Action of Calotorpis procera on migraine (Family Asclepiadaceae), J. National integrated Medical association, 27, 7-10.

51. Radhakrishan, K., Pandurang, A.G. and Pushpanganda, P. 1998. Uteria salicifolia, A new ethnobotanical record from Kerala, India, Fitoterapia, 69(5): 403-405.

52. Rai, M.K., Pandey, A.K. and Acharaya, D. 2000. Ethnomedicinal plants used by Gond tribe of Bahanalehi District Chhinwara, M.P. J. of nontimber forest, 7(3/4): 237-241.

53. Rasik, M. Raghubir, R. Gupta, A. Shukla, A., Dubey, M.P., Srivastava, S., Jain, H.K. and Kulshreshta, D.K. 1999. Healing potential of C. procera on dermal wounds in Guinea pigs, J. Ethnopharmacol., 68 (1-3): 261-266.

54. Reddy, V.B. 2008. Use of various bio fencing plants in the control of human diseases by Lambda Tribe Inhabiting Nagaland District, A.P., India, Ethnobotanical Leaflets, 12, 520-523.

55. Rehman, E.U. 2009. Indiginous knowledge on medicinal plants , Village Baralikass and its allied areas, District Kotli Azad, Jammu and Kashmir, Pakistan, Biodiversity Network J & K, .

56. Said, H.M., Barkati, M.A. & Khalil, A.K. 1996. Mufeed Ghizaen Dawain, Bai-tal Hikmat, Mohammad Bin Qasim Avenue, Madinat -al-Hikmat, Karachi-74700, 10-14.

57. Sen, S.K. and Behra, L.M. 2007. Ethnomedicinal Plants used in touch therapy at Bargarh district of Orissa, Ethnobotany, 19, 100-104.

58. Shah, S.R.U., Hassan, G., Rehman, A. and Ahmed, I. 2006. Ethnobotanical studies of the flora of the district Musakhel and Barkhan in Balochistan. Pakistan, Pak. J. Weed Sci. Res. 12(3): 199-211.

59. Sher, H. and Hussain, F. 1998. Income generation from the trade and cultivation of medicinal plants for local communities in district Swat, Pakistan. Proc: Wild plants resources of Northern Pakistan, workshop PFI, Peshawar May 11-12, 1998a, 48-51.

60. Shinwari, M.I. and Khan, M.A. 2000. Folk use of medicinal herbs of Margalla hills national park, Islamabad, J. Ethnopharm. 69, 45-56.

61. Showkat, R.M. 2007. Different system of classification of crude drugs,Alternate system of medicine, Pharmacognosy.

62. Smith, H.F., Woerdenbag, H.J., Singh, R.H., Meulenbeld, G.J., Labadie, R.P. and Zwaving, J.H. 1995. Ayurvedic herbal drugs with possible cytostatic activity, J. Ethnopharm, 47, 75-84.

63. The Wealth of India, Raw Materials, Vol. II, CSIR, New Delhi, 1959, 20-23.

64. Vaidya, A. 1998. Pharm. Res. India (Pharm a Pulse- Suppl), 44-45.

65. Zabihullah, Q. Rashid, A. and Akhtar, N. 2006. Ethnomedicinal survey in Kot Manzorag Baba Valley Malakand Agency, Pakistan, Pak. J. Pl. Sci. 12(2): 115-121.