Ethnobotanical Leaflets 12: 693-97. 2008.
Phytochemical Evaluation of Certain Anticancer
Crude Drugs Used by Adivasis of Rayalaseema
P. Venkata Krishnaiah, K. Venkata Ratnam2 and R.R.Venkata Raju¹*
*Corresponding author: Prof. R.R.Venkata Raju, Department of Botany,
E- mail: firstname.lastname@example.org
Phone: 918554 255726 (O); 09440289488 ( Mobile)
The present paper deals with
preliminary phytochemical evaluation of certain
crude drugs used for cancer. Based on the folklore data obtained from adivasis of Rayalaseema region,
Andhra Pradesh, 15 species were collected in the forests of
Key words: Cancer, Crude drugs, Secondary metabolites.
region lies between 1241'-1621' N latitudes and 1745' - 8110'
E longitudes. The area accounts 26% of the total area of the Andhra Pradesh
state and consists of four districts viz.,
In every ethnic group there exists a traditional health care system, which is prevalent and popular among the community. The indigenous society has always been associated with nature for their needs with respect to health care system. The tribal communities have given first and foremost importance to their health care. The critical review of literature revealed that very little attempts were noticed (Reddy, 2008) on remedies for cancer; hence the present paper gains importance.
The folklore information was recorded from the local tribal practitioners and from the tribal healers by conducting repeated interviews. Based on the recorded folklore information 15 species were selected as widely used crude drugs for cancer. The voucher specimens were prepared and identified with the help of local / regional floras (Gamble, 1915 – 35; Venkata Raju and Pullaiah, 1995) and confirmed by comparing with authenticated specimens housed at Sri Krishnadevaraya University Herbarium (SKU), Anantapur.
The samples were collected in bulk quantities for preliminary phytochemical screening. The collected samples were shade dried, powdered about (100g) and successively extracted with ethanol using soxhlet apparatus for 6 hours. The extracts were filtered and concentrated under reduced pressure to dryness. The extracts were subjected for preliminary phytochemical screening using standard procedures (Amarasingham et al., 1964, Das and Bhattacharjee 1970, Gibbs, 1974 and Harborne, 1991).
The present paper provides information on preliminary phytochemical evaluation of certain anticancer crude drugs. The solvent extracts of the drug yielding plants were subjected for preliminary phytochemical screening. The positive reactions were observed for 17 different groups of secondary metabolites. The distribution and richness of different constituents were statistically analyzed (Table-1). The frequency and distribution of different chemical constituents were analyzed (Table – 2).
Table 1. The distribution and richness of different constituents in crude drugs used for cancer.
Al = Alkaloids; An = Anthocyanins; Ad = Anthocyanidins; At = Anthracene glycosides; Au = Aucubins; Aq = Anthraquinones; C = Catecholic Compounds; Co = Coumarins; F = Fatty Acids; Fv = Flavonoids; Gt = Gallic Tannins; I = Iridoids; P = Phenols; S = Saponins; St = Steroids; Tt = Triterpenoids; V = Volatile Oils.
The preliminary phytochemical analysis revealed that, coumarins and flavonoids were recorded as most common group of compounds in majority of the test samples (14 spp each, 93.3%) followed by alkaloids and anthracene glycosides (12 spp each, 80%), triterpenoids (11 spp, 73.3%), iridoides and saponins (10 spp each, 66.6%) while aucubins were noticed in one species (6%) only. Flavonoides, the major group of phenolic compounds reported for their antimicrobial (Toda et al., 1989), antiviral (Barnard et al., 1993) and spasmolytic (Amor et al., 2005), while coumarins reported for antibacterial (Fernanden et al., 1996) and antifungal (Houet and Paya, 1996)
The evaluation on the occurrence and distribution of various phytochemical compounds revealed that maximum number were found in Curculigo orchioides (12) followed by Terminalia chebula (11), Cassia fistula (10), Plumbago zeylanica and Dregea volubilis (9each), Aristolochia indica, Entada pursaetha, Lannea coromandelica and Semecarpus anacardium (8each), Andrographis paniculata, Buchanania lanzan, Datura metel and Rhinacanthus nasutus (7each), Tinospora cardifolia (5) while minimum compounds were noticed in Anogeissus latifolia(4).
Table 2. The frequency and distribution of different chemical constituents were analyzed.
The critical analysis of the data in the light of literature (Jain, 1991; Kirtikar and Basu, 1935) resulted the formation of drugs and mode of administration for 3 drug yielding plants were recorded first time, hence indicated with asterisk. The comprehensive data is provided to indicate the distribution of various groups of compounds in the respective solvent extracts. The fractionation and characterization of active principle involved in healing property is being attempted in the laboratory.
The authors are thankful to the conservator of forests and other officials of Andhra Pradesh forestry for permission and help during exploration trips.
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