Ethnobotanical Leaflets 13: 702-08, 2009.
Effect of Berberis lycium Royle on Lipid Profile in Alloxan Induced Diabetic Rabbits
Maqsood Ahmed1, Alamgeer1, Tanveer Sharif 2, Muhammad Zabta CH3 and Atif Akbar4
2Department of Pharmacy, G C University,
3Faculty of Pharmacy,
*Correspondjng author: Email: email@example.com
Berberis lycium Royle (B.l.R) commonly known as Ishkeen (Kashmal and Darbald) is widely used in folk medicines for the treatment of Diabetes mellitus. Experimental diabetes use to alter the lipid profile. The aim of the present study was to evaluate the effects of Berberis lycium root bark on various lipid profiles in alloxan induced diabetic rabbits. Diabetes was induced by single intravenous injection of Alloxan (150 mg/kg).Oral administration of 250mg/kg and 500mg/kg crude powder of Berberis lycium root for four weeks resulted in significant reduction in total cholesterol, triglyceride and low density lipids (LDLs) levels. Berberis lycium treatment increased the levels of high density lipids (HDLs). Furthermore same doses stabilized the weight of diabetic rabbits. Thus our investigation clearly shows that crude powder of Berberis lycium Royle has antihyperlipidemic effect.
Key words: Berberis lycium Royle; root bark; lipid profile; diabetes.
Traditional herbs and plants have been used for the treatment of various diseases, Nadkarni (1954). Berberis lycium Royle is traditionally used medicinal plant for treating various diseases including diabetes mellitus particularly by the local inhabitants of Hamaliya region, (Muhammad et al., 2006). Induced diabetes use to alter the lipid profile in alloxanized diabetic rabbits (Wojtowicz et al., 2004, Maciejewski et al., 2001). The aim of the present study was to evaluate the antihyperlipidemic effects of Berberis lycium in alloxan induced diabetic rabbits.
Berberis lycium Royle is a shrub which belongs to
family Berberidaceae and is commonly known as Ishkeen (Local name in shina
language). It is commonly found in
Materials and Methods
Berberis lycium cortical part of
root was collected in the month of April,2007 from the shrubs, growing in
village Shikyote District Gilgit of Hamaliya region. Plants were identified
and authenticated by Dr. Bukhari, Department of Botany (Ex Director)
The serum total Cholesterol, Triglyceride, HDLs and LDLs, were evaluated by enzymatic test kits (Randox).
LDLs level was calculated by using following, (Fridewald et al.,1979)
Male albino rabbits belonging to the local strain (Oryctolagus cuniculus) with average weight 1.21 Kg and a range of 1.0-1.5 Kg were used.
Treatment of Rabbits
All animals were housed at the animal house of Department of Pharmacy Bahauddin Zakariya University Multan .Animals were housed in stainless cages under standard laboratory condition (light period 8.00 A.M. to 8.00P.M. 21±2oC, relative humidity 55% , green fodder (Medicago sativa) and water available ad labitum . Animals received human care. The study protocol was approved by the local ethical committee.
Induction of diabetes and experimental design
divided into three major experimental groups (normal control (a), diabetic
control (b) and diabetic with Berberis
lycium bark powder treatment). Experimental rabbits were further
subdivided into two groups (c) Receiving 250 mg/kg body weight (d) 500 mg /kg
body weight crude powder of root bark. At the start of the experiment the
animals in the later two groups were injected intravenously with 150 mg/kg of
10% alloxan monohydrate (
Effect of Berberis lycium Royle on lipid profile in alloxanized rabbits
Rabbits were treated with 250 mg/kg body weight and 500 mg/kg body weight crude root powder for one month. Blood samples of all groups were taken at 0 day and 30th day and different lipid parameters were studied.
Effect of Berberis lycium Royle on weight of rabbits
To check the effect of Berberis lycium Royle on weight of alloxanized rabbits Weight of rabbits was measured at o day, 6th day and 30th day in different experimental groups
The data was expressed as mean ± standard deviation (SD) and analyzed using analysis of variance (ANOVA). (p<0.05) was considered as significant.
Results and Discussion
Table 1: Effect of Berberis lycium Royle root bark powder on plasma cholesterol, triglyceride, LDLs and HDLs in alloxan-induced diabetic rabbits for 1 month.
Group Total cholesterol Triglyceride LDLs HDLs
(mg/dl) (mg/dl) (mg/dl) (mg/dl)
Normala 44.6±4.17 53.6±2.80 5.88 ±0.12 28±3.14
Diabetic b 64.20±2.42 123.4±30.7 16.92 ±7.6 22.6±2.07
250 mg/kg c 51.21±2.88 * b 60.3 ±5.37** b 9.55 ±1.59* b 29.6±2.3
500 mg/kg d 49.5±2.60** b 58.2±18.2** b 7.06±2.93 * b 30.80±2.28* b
Values are expressed as Mean ± SD n=5 rabbits in each group a-d shows different groups and estaric indicate significant difference (*P < 0.05:**P < 0.01 ) compared to group b
Effect on weight of rabbits
A significant decrease (P<0.05; P<0.001) in weights of diabetic rabbits was observed when the weights measured at 0 day, 6th day and 30th day were compared. While weights of treated diabetic rabbits receiving 250 mg/Kg and 500 mg/Kg body weight of Berberis lycium root bark powder were stabilized, when the weights of treated diabetic rabbits measured at 6th day and 30th day were compared. Weights of control, diabetic and treated groups are shown in (Table 2).
Table 2: Effect of Berberis lycium Royle root bark powder on weight in alloxan induced diabetic rabbits at 0, 6th and 30th day.
Group Weight (Kg)
At 0 day At 6th day At 30th day
Diabetic (b) 1.41± 0.8 1.23± 0.74* 1.04±0.65**
250 mg/Kg (c) 1.25 ± 0.39 1.19±0.45* 1.19±0.12*
500 mg/Kg (d) 1.51 ± 0.14 1.49±0.98 1.43±0.52
Values are expressed as Mean ± SD n=5 rabbits in each group a-d shows different groups and estaric in the same row indicate significant difference (*P<0.05; **P<0.01) compared to 0 day.
present study it was observed that crude powder of Berberis lycium root has anti-hyperlipidemic effect in
alloxanized rabbits. Hypercholesterolemia and hypertriglyceridemia have been
reported to occur in alloxan induced diabetic rabbits, (Wojtowicz et al. 2004; Maciejewski et al. 2001). These effects are in
line with present study. Berberis
lycium Royel root bark powder significantly reduced the total cholesterol
and triglyceride and
demonstrate that Berberis lycium royle
root bark powder contained anti hyperlipedemic activity in allaoxanized
rabbits it decreased the elevated level of total cholestrole ,Triglicerides,
Batteridge, J. and Willams, J.C.G (eds)
1997. Lipid disorders in diabetes mellitus. Text Book of Diabetes
2. Chand, N. Durrani, F.R. Qureshi M.S. and Durrani, Z 2007. Role of Berberis lycium in Reducing Serum Cholesterol in Broilers. Asian Aust J Anim Sc 4 : 563-568.
3. Fridewald, W.T. Levy, R.I. and Sloane – Stanley, G.H 1979. Estimation of concentration of Low density cholesterol in plasma, without use of preparative ultracentrifuge. Clinc.Chem 18: 499-504.
Rang, H.P.Dale, M.M. Ritter, J.M. and
5. Janbaz, K.H. and Gilani, A.H. 2000. Preventive and curative effect of Berbarine in hepatotoxic rodents. Fitoterapia 71: 25-33.
7. Laakso, M. 1995. Diabetic Epidemiology of diabetic dyslipidemia. Diabetes Rev 3: 408-410.
8. Maciejewski, R. Rucinski, P. Burski, K. and Figura, T. 2001. Changes in glucose, cholesterol and serum lipid fraction levels in experimental diabetes. Ann Univ Mariae Curie Sklodowska 56: 363-368.
9. Muhammad, W. Muhammad, A.U.S. Rizawan, A.Q. Iqbal, M. Rabia, A. and Saeed, Y. 2006. Traditional uses of various plants of northern areas. Acta Botanica Yunnanica 28: 535-542.
Nadkarni, K.M. 1954. In: Indian Materia medica 2nd
ed.. Popular Book Depot,
11. Shamas, F. Ahmadiani, A. and Khosrokhavar, R. 1999. Anticholinergic and antihistaminic activity of Berberis fruit. J Ethanopharmaco 64:161-166.
13. Way, K.J. Kata, I.N. and King, G.L 2001. Protein Kinase C and the development of diabetic vascular complication. Diabet. Med 18: 945-950.
Wojtowicz, Z. Wrona, W. Kis, G
Blaszczak, M. and Solecka, A.
2004. Serum total cholesterol, triglyceride and High density