Ethnobotanical Leaflets 9: 6-14. 2005.
Comparison in Various Bioactive Compounds of Leaves and Seeds of Foeniculum Vulgare Mill.
Gulfraz, M*., M. Arshad*, N. Uzma**, Kanwal** and K. Shabir**
* Associate Professor, Department of Biochemistry
** M.Sc. Students, Department of Biochemistry
Medicinal plants a gift
of nature are being used against various infections and diseases in the
subcontinent since past history. Herbs were use as food ( vegetables ) and flavors for hundred of years in many
parts of world. Whereas number of herbs have also been traditionally regarded
as natural remedies for common ailments of human population. Furthermore some
herbal plants are considered as house of medicines and played an important
role in nearly every culture on earth, including
Key words ; Foeniculum vulgare, medicinal plants, chemical analysis.
Approximately 80% of the words population depends exclusively on plants for their health and healing. Where as in the developed world, reliance on surgery and pharmaceutical medicine is more usual but in the recent year, more and more people are complementing their treatment with natural supplements. (Dursum et al. 2004). Furthermore motivation of people towards herbs are increasing due to their concern about the side effects of drugs, those are prepared from synthetic materials. The people want to concern their own health rather than merely submitting themselves to impersonal health care system. Many botanicals and some common dietary supplements are good sources of antioxidants and anti-inflammatory compounds (Leung and Foster, 1996: Nadkarni, 1976)
Foeniculum vulgare belongs to family Apiaceae, which is an annual, biennial or perennial aromatic herb, depending on the variety ( Farrell, 1985: Wichtl and Bisset, 1994). It is a source of Phytochemicals hormones (saponins), Proteins, amino acids, fats, flavonoids and many other organic compounds .while all of these organic compounds are important for various purposes of human population. The use of these secondary compounds of plants for the treatment of human�s ailments is indeed very old ( Aritomi and Kawasaki, 1984 : Miura et al. 1986 ).
Foeniculum vulgare commonly known as fennel and develops an edible bulb contained thick base leaves, which is becoming increasing popular as a vegetable. The leaves, stalks and seeds (fruits) of the plant species are edible. It is used as carminative, digestive, lactogogue and diuretic (Matin et al, 2002). However, Foeniculum vulgare is an aromatic herb whose fruits are oblong, ellipsoid or cylindrical, straight or slightly curved and greenish or yellowish brown in colour. The weight of between 6 and 7 mg where as length is 6 mm and width 2mm. The dried, aromatic fruits are widely employed in culinary preparations for flavoring bread, pastry and candies. It is also use in alcohol liqueurs, as well as in cosmetic and medicinal preparations. (Bernth, 1998: Matin et al, 2002). This herb has finely out feathery foliage, umbels of mid summer flowers, curved, ribbed seeds and a thick root. It is used as an expensive and extravagant spice and vegetable in different parts of world. Its seeds contain essential oil, which is used for many purposes by human population (Tanira, 1996: Leung and Foster, 1996).The oil of Foeniculum vulgare regulates the peristaltic functions of the gastrointestinal tract, thereby reducing emptying time and increasing the passage of gas. It also relieves the spasm of intestines. It was experimentally observed that Foeniculum seeds are effective against hernias and hydrocele when used with other salts or ingredients. (Fathy et al. 2002 : Ethernton et al. 2002 ).
Therefore keeping in view the importance of Foeniculum vulgare the present study was undertaken with following aims and objectives.
. To analyze bioactive compounds in leave and seeds of Foeniculum vulgare.
����������� To assess the Foeniculum leave and seeds for Saponin and Flavonoids.
����������� To highlight effects of Foeniculum on health of human population.
Collection and Preparation of Leave and Seeds Samples
The leave and seeds samples of Foeniculum vulgare
were analysed for various organic compounds in this
study. The leaves and seeds were collected from different hilly areas nearby
Analysis of Total Protein, Amino acid and Lipids from Leaves and Seeds of Foeniculum vulgare
The total protein content of leaves and seeds were determine by using kjeldahal method whereas total lipid content were determined by taking ether extraction by soxhlet apparatus whereas percentage of lipid was determined by calculating initial and final weight of samples.
Analysis of Protein and Amino acids by Thin Layer Chromatography
The purified samples of leaves and seeds of Foeniculum vulgare were subjected to one and two dimensional thin layer chromatography. Furthermore the Rf values of each amino acid was calculated and compared with standard values.
Analysis of Flavonoids and Saponins
The analysis of flavonoid and saponins from leaves and seeds of Foeniculum were carried out in the column chromatography by using silica gel. The columns were washed with various solvents ( Diethyl ether,n-propanol,ethanol and methanol etc.) on the basis of their polarity order.( Aritomi and Kawaski,1984: Gulfraz et al. 2004). Furthermore 100 grams of samples has provided about 10 grams of purified (Flavonoid or Saponin ) compounds.
Furthermore all chemicals used in this study were analytical grade (Sigma and Merck ) and were imported from abroad.
The results of flavonoids, saponins, proteins, amino acids, fats etc are given in the tables 1-6. Whereas comparison of various compounds in both leaves and seeds are given in figures 1-2.
Results obtained after analysis of leaves and seeds of Foeniculum vulgare indicates that flavonoids , saponins, proteins, amino acid and fats were present in these samples.The leaves contained higher concentration of flavonoids and fat. Whereas the level of saponins, protein, amino acids and other organic compounds were high in seeds. (Tables 1-6 and Figs 1-2) . The seeds of Foeniculum are considered as essential ingredients for many local medicines those can used against stomach, kidney and liver infection and disorders. (Etherton, 2002: Matin et al. 2002). The organic compounds obtained from seeds will further increase the market values of these valuable medicinal plants. The younger and fresh leaves are consider as delicious and traditional vegetables in many areas of this region (Karnick,1998) . Furthermore Seeds (fruits) are being use in all most all of houses of this region for many purposes of human population, whereas leaves are mostly used as vegetables either cooked or in the form of salad.( Zaidi,1998 )
Figure 1. �Phytohormones (Saponin) extracted from seed of Foeniculum Vulgare.
Figure 2. �Flavonoids extracted from leaf of Foeniculum Vulgare.
Figure 3. �Proteins extracted from seeds of Foeniculum Vulgare.
Table 1. The Percentage (%) of different chemical compounds in leave and seeds of
Table 2 . The Percentage (%) of Saponins in leave and seeds of Foeniculum Vulgare.
Table 3. The Percentage (%) of Flovonoids in leave and seeds of Foeniculum Vulgare.
Table 4. The Percentage of Amino acids in leave and seeds of Foeniculum Vulgare.
Table 5. Spectrophotometeric analysis ( 470 nm) of leave of Foeniculum Vulgar after extraction of
various compounds with different solvents.
Table 6. Spectrophotometeric analysis ( at 470 nm) of seeds of Foeniculum Vulgare after extraction of
various compounds with different solvents.
Figure 1:- Comparison in concentration, absorbance and pH values of saponins analyzed from
Seeds of Foeniculum vulgare with different solvents.
Figure 2:- Comparison in concentration, absorbance and pH values of Flavonoids analyzed
from leaves of Foeniculum vulgare with different solvents.
� Aritomi, M and T. Kawaskaki, 1984. Three highly oxygenated Flanone Glucuronides in
�Leaves of Spinacia Oleracea. Phytochemist.23, 2043-47.
� Bernth, J. 1998.. Biological and structural regularities of essential oil accumulation in
�developing fruits of Foeniculum
vulgare Mill) , Med. Pl.prod.
E, S. Otles and E. Akcicek.
2004. Herds as Food source in
�Cancer Prev 5. 334-339.
� Etherton, P. H, K. Hecker and A. Bonomone. 2002. Bioactive compounds in foods: Their
�role in Prevention of cardiovascular disease and cancer. Am. J. Med. 113, 71-88.
� Karnick, C.H. 1994. Pharma copocial standards of Herbal plants,Sri, Satg.Pub,(1-2) 139-141.
K. T. 1988. Spices, Condiments and Seasonings.
� Fathy, M., S. Afaf. H. Shehata, A. E. Kaleel and S. M. Ezzhat. 2002. An acylated Kaempferal
����� Glycoosider from Flovones of Foeniculum vulgare and F. Dulce. Moecules, 7, 245-251.
� Gulfraz, M., M. Arshad,k N. Nayyar, N. Kanwalj and U. Naisr.2004. Investigation of Bio active compounds of Berberis lyceum Royal and Justicia Adhatoda �������� L. Ethanbotanical. Leaflet. world wide web, 1-10.
A.Y and S. Foster. 1996. Encyclopedia of common Natural Ingredients used in
food, Drugs, and cosmetics, (ed) Rube.John
� Matin, A., M. khan, A.Ashraf and R.A. Qureshi.2002.Tradational use of shrubs and trees of Himalayan Region, Shogran Valley, Distrist Mansehra (Hazara), ��������� Pakistan.Humdard Medicus.XLV(2):51.
� Miura, Y, K.k Ogawa, H. Fukuir and M. Tabata. 1986. Changes in the essential oil components
�during the development of Fennel plants from somatic Embryokides. Plant Med. 52, 96-5.
Nad Karni, K. M
1976. Indian Materia Medica
� Simon, J.E and A. Quinn 1988. Cheracterization of essential oil of parsley. J. Agr, Food chem.. 36 : 467-472.
� Tanira, M.O.M, 1996. Phaemacological and toxicological investigation on Foeniculum vulgare dired fruit extract in experimental animals. Phytother Res 10:33-36.
M and N.G. Bisset 1994. Herbal drugs and phytopharmaceuticals (ed), Med. Pharm scientific Publ
S. H. 1998. Existing indigenous medicinal plant resources of