Ethnobotanical Leaflets 12: 912-15. 2008.
Antimicrobial Studies of Triterpenoid Fractions from Myxopyrum smilacifolium Blume
Sudharmini, D.* and Ashalatha S. Nair
*email : email@example.com
����� Triterpenoids isolated from Myxopyrum smilacifolium leaf showed presence of ursolic acid (0.175mg/g). The triterpenoids showed antimicrobial activity in gram positive bacteria and Candida sps.
Keywords: Myxopyrum smilacifolium,Ursolic acid, HPLC, Antimicrobial activity.
Myxopyrum smilacifolium (Oleaceae) is an important medicinal plant
widely used in indigenous system of medicine in
2. Use in traditional medicine
��������� The leaves are useful in vitiated conditions of kapha and vata, cough, asthma, rheumatism, cephalalgia, nostalgia, fever, otopathy, neuropathy and cuts and wounds. Roots are useful in scabies and prurigo in children 
3. Previously isolated classes of constituent
Iridoid glucosides, namely myxopyroside .
4. New constituents
������� Isolation of triterpenoid fractions by silica gel thin layer chromatography using the mobile phase� petroleum: dichloroethylene: acetic acid (50:50:0.7) ., HPLC analysis of ursolic acid and antimicrobial activity by using filter paper disc diffusion method [4, 5].
5.� Used materials
������� Ursolic acid standard was collected
from Sigma, Aldrich,
Reported in figure1 and 2, and� Table1.
Ursolic acid is found in the leaves of Myxopyrum smilacifolium. All the tripterpenoids from the plant showed antimicrobial activity against the gram positive bacteria Staphylococcus aureus, Bacillus subtilis, Candida� albicans and C. glabrata. The antifungal activity was similar to the chemical fungicide.
��������� The first author is thankful to
University Grant Commission (UGC), Government of India,
 Varier P S, Indian medical
plants: a compendium of 500 species, vol.4.
 Franzyk H, Jensen S R, Olsen C E. J Nat Prod 2001; 64:632-633.
 Harborne J B. Phytochemical Methods: A
guide to modern technique of plant analysis. 3rd ed.
 Liu C H., Liu Y. China journal of Chinese materia medica 2002; 27(12):916-918.
Benson H J. Microbiological applications. 5th ed.
Table 1. Antimicrobial activities of isolated triterpenopid fractions of
������������ Myxopyrum smilacifolium.
Values are the mean of three replicates.������� TTR*- Triterpenoid����������������
a �Including the diameter of the filter paper disk (6mm).
Fig 1. Ursolic acid HPLC profile at 205nm.
Fig 2. Myxopyrum smilacifolium� fraction (hRf 20) HPLC profile.